Hello there...
Today we will learn some important practical points about CLED.
The first very common question that a brooding examiner will ask you is..
What is the full form of CLED.
The answer to this lame question is pretty much there in the title.
What is CLED used for?
Well CLED is most importantly and commonly used for isolation and enumeration of urinary pathogens.
What is the C source??
Not to worry, it is there in it's name itself. Lactose is the C source used.
Hearing your this question, another question that will be bombarded on you will go something like this.
Oh really, is cystine then added for you to grow in it?
(To which you must proudly answer) Obviously no, L- cystine is incorporated in the medium to support growth of cystine depending dwarf coliforms. Incorporation of cystine makes CLED stand out from other medias and is thus known to promote growth of urinary pathogens.
(P.S. Do not forget to tug on your collar after giving this answer, attitude dude).
What is the indicator used?
Bromothymol blue - turns yellow in acidic condition and pink or deep blue in alkaline conditions.
What do you mean by electrolyte deficient?
No electrolyte is added in the medium to inhibit swarming of Proteus, hence the medium is electrolyte deficient.
Examples of both the changes?
E.coli and k.pneumoniae - gives yellow colored colonies.
P.vulgaris and S.typhi - gives blue colored colonies.
Hope it helps you.
Do your best.
Keep learning and be amazing <3
-Staph.
Today we will learn some important practical points about CLED.
The first very common question that a brooding examiner will ask you is..
What is the full form of CLED.
The answer to this lame question is pretty much there in the title.
What is CLED used for?
Well CLED is most importantly and commonly used for isolation and enumeration of urinary pathogens.
What is the C source??
Not to worry, it is there in it's name itself. Lactose is the C source used.
Hearing your this question, another question that will be bombarded on you will go something like this.
Oh really, is cystine then added for you to grow in it?
(To which you must proudly answer) Obviously no, L- cystine is incorporated in the medium to support growth of cystine depending dwarf coliforms. Incorporation of cystine makes CLED stand out from other medias and is thus known to promote growth of urinary pathogens.
(P.S. Do not forget to tug on your collar after giving this answer, attitude dude).
What is the indicator used?
Bromothymol blue - turns yellow in acidic condition and pink or deep blue in alkaline conditions.
What do you mean by electrolyte deficient?
No electrolyte is added in the medium to inhibit swarming of Proteus, hence the medium is electrolyte deficient.
Examples of both the changes?
E.coli and k.pneumoniae - gives yellow colored colonies.
P.vulgaris and S.typhi - gives blue colored colonies.
Hope it helps you.
Do your best.
Keep learning and be amazing <3
-Staph.
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