Tuesday, 4 August 2015

Difference between Mixed acid fermentation pathway and Butandiol pathway

Mixed  Acid  Fermentation
Butandiol Fermentation
Hello everyone,
Today with Staph you will be learning how to distinguish between mixed acid fermentation and butandiol pathways.
Hope it helps :)

Mixed acid fermentation
Yield: 3 ATPs per glucose molecule



Butandiol fermentation
2 ATPs per glucose molecule.
Only one decarboxylation reaction is observed in the pathway catalysed by formate hydrogen lyase.

Three decarboxylation reaction are observed in the pathway catalysed by formate hydrogen lyase, α-aceto-lactate synthase, α-aceto-lactate decarboxylase.
Acidic end products-causes reduction in pH of the medium to 4.5 from near neutral.
Alcoholic end products-pH of the medium remains to 6.
Organisms following this pathway gives a strong methyl red test
Organisms following this pathway gives a very strong vogus prausker test
End products-Succinate, formate, lactate, acetate, ethanol, CO2 and H2.
End products-           lactate, formate, ehtanol, 2,3 butandiol, CO2 and H2.
Orgainsms- E.coli, Salmonella, Shigella
Klebsiella, Enterobacter, Serratia, Erwinia

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