Hello amigos :D
Welcome to Microamaze.
Today we are learning principle behind oxidase . The protocol is quite easy and hence we will not be going in that lane.
So with no going off topic at all today, let's begin and get over with it as fast as possible.
Oxidase test is one of the most known Rapid tests and is used to differentiate or identify the micro-organisms which that respire aerobically or use oxygen as the final electron acceptor.
Cytochrome oxidase is the common oxygen utilizing enzyme and reduces it at the end of the electron transport chain. The reagent that we use here is tetramethyl-para-phenylenediamine dihydrochloride or it is dimethyl-para-phenylenediamine dihydrochloride.
I know the name seems very monstrous but there is no other way to remember it unless you are Memory girl from Pokemon Generation VI :p
So this tetra thing is a colorless redox reagent which contains a chromogenic reducing component and acts like an artificial oxygen acceptor. It changes color to dark blue or purple when it gets oxidised and that colored compound is Pumpkaboooooo...
-_- WTF?
Oh no, it's Indophenol Blue or Wurster's Blue.
Hey, there is a sauce called Wooster sauce :p
Anyways, so if this colored compund forms within ten sex , I mean ten secs of the reaction between the reagent and the colony, the test is concluded to be positive.
That's pretty much of the principle.
I hope it helps :)
We will end the post with a high six.
What is that?
Well it's something between me and my readers.
A virtual internet high five is doing around too much these days. So this is my virtual high six.
Lots of love
-Staph with greater than three. <3
Welcome to Microamaze.
Today we are learning principle behind oxidase . The protocol is quite easy and hence we will not be going in that lane.
So with no going off topic at all today, let's begin and get over with it as fast as possible.
Oxidase test is one of the most known Rapid tests and is used to differentiate or identify the micro-organisms which that respire aerobically or use oxygen as the final electron acceptor.
Cytochrome oxidase is the common oxygen utilizing enzyme and reduces it at the end of the electron transport chain. The reagent that we use here is tetramethyl-para-phenylenediamine dihydrochloride or it is dimethyl-para-phenylenediamine dihydrochloride.
I know the name seems very monstrous but there is no other way to remember it unless you are Memory girl from Pokemon Generation VI :p
So this tetra thing is a colorless redox reagent which contains a chromogenic reducing component and acts like an artificial oxygen acceptor. It changes color to dark blue or purple when it gets oxidised and that colored compound is Pumpkaboooooo...
-_- WTF?
Oh no, it's Indophenol Blue or Wurster's Blue.
Hey, there is a sauce called Wooster sauce :p
Anyways, so if this colored compund forms within ten sex , I mean ten secs of the reaction between the reagent and the colony, the test is concluded to be positive.
That's pretty much of the principle.
I hope it helps :)
We will end the post with a high six.
What is that?
Well it's something between me and my readers.
A virtual internet high five is doing around too much these days. So this is my virtual high six.
Lots of love
-Staph with greater than three. <3
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